Bromelain
Bromelain Powder Manufacturer | High Activity Enzyme 2400 GDU/g (CAS: 9001-00-7) Headline: Bromelain Enzyme Powder | Natural Proteolytic Power from Pineapple (CAS: 9001-00-7) Sub-info: Activity 1200 GDU/g - 3000 GDU/g | Extracted from Pineapple Stem | Non-GMO & Halal/Kosher. USP: • High Potency: Standardized activity up to 2400 GDU/g (Gelatin Digestion Units) for maximum digestive support. • Systemic Anti-Inflammatory: Clinically proven to reduce swelling and inflammation in joints and soft tissues. • Cold-Chain Process: Extracted via ultra-filtration and freeze-drying to preserve enzymatic activity and stability.
Introduction
Bromelain (CAS: 9001-00-7) is a mixture of proteolytic enzymes (cysteine proteases) found naturally in the pineapple plant (Ananas comosus). While it exists in the fruit, commercial-grade Bromelain is extracted from the pineapple stem, which contains a higher concentration of heat-stable enzymes.
We utilize advanced membrane separation and vacuum freeze-drying technologies to produce a High-Activity Bromelain Powder. Unlike solvent-precipitated versions, our product maintains a high specific activity and is widely used in Dietary Supplements (Joint & Digestion), Functional Foods (Meat Tenderizing), and Cosmetics (Exfoliation).
Category：
Keywords：
bromelain
activity
Functions
1.Protein Digestion (Digestive Aid)
Bromelain hydrolyzes high-molecular-weight proteins into smaller peptides and amino acids. It functions effectively across a wide pH range (4.5 - 9.8), making it an excellent digestive aid that works in both the stomach and small intestine to relieve bloating and indigestion.
2.Anti-Inflammatory & Joint Support
Systemically, Bromelain inhibits the production of pro-inflammatory kinins and reduces the migration of neutrophils to sites of injury. It is widely prescribed in Europe for reducing post-surgical swelling (edema) and alleviating Osteoarthritis pain.
3.Biofilm Disruption
Bromelain has been shown to hydrolyze the peptide bonds in the biofilm matrix produced by bacteria, enhancing the effectiveness of antibiotics and supporting immune defense.
Applications
1.Dietary Supplements
• Joint Formulas: Often combined with Turmeric (Curcumin) and Boswellia for a potent natural pain-relief stack.
• Digestive Enzymes: Blended with Papain, Amylase, and Lipase for comprehensive digestive support capsules.
2.Food Industry
• Meat Tenderizer: Hydrolyzes collagen and elastin in muscle fibers, making meat softer and more palatable without leaving a mushy texture.
• Baking: Improves dough relaxation and texture in crackers and biscuits by breaking down gluten.
3.Cosmetics & Skincare
• Enzymatic Peels: Gently digests dead skin cells (keratin) on the surface, offering a non-abrasive alternative to AHAs for brightening and smoothing skin texture.
Flow Chart
Physical Separation to Preserve Bioactivity
1.Raw Material: Fresh Pineapple Stems (Harvested post-fruit collection).
2.Juice Extraction: Pressing stems to release enzyme-rich juice.
3.Filtration: Removing fibers and suspended solids.
4.Ultra-Filtration (Concentration): Using molecular weight cut-off membranes to concentrate the enzyme protein.
5.Purification: Removing impurities and small molecules.
6.Micro-Filtration: Bacteria removal (Cold Sterilization).
7.Freeze Drying: Low-temperature drying to prevent protein denaturation.
8.Standardization: Blending to target GDU (e.g., 2400) with maltodextrin carrier.
9.QC Testing: Activity Assay (GDU/g) & Heavy Metals.
10.Packing: Aluminum Foil Bags in Drums.
Quality Standard of Lactoferrin
Product: Bromelain 2400 GDU/g (Food/Pharma Grade)
| Item | Specification | Result |
| Appearance | Off-white to light yellow powder | Conforms |
| Odor | Characteristic pineapple aroma | Conforms |
| Enzyme Activity | ≥ 2400 GDU/g | 2450 GDU/g |
| Protein Content | ≥ 80% | 85% |
| pH (1% Solution) | 4.5 ~ 6.5 | 5.2 |
| Loss on Drying | ≤ 5.0% | 3.1% |
| Ash | ≤ 3.0% | 1.2% |
| Heavy Metals (Pb) | ≤ 5.0 ppm | Conforms |
| Arsenic (As) | ≤ 2.0 ppm | Conforms |
| Total Plate Count | < 3000 cfu/g | < 500 cfu/g |
| Salmonella / E.Coli | Negative | Negative |
Method of Analysis of Lactoferrin
GDU (Gelatin Digestion Unit) Assay
• Definition: One GDU is the amount of enzyme that will liberate 1 mg of amino nitrogen from a standard gelatin solution after 20 minutes of digestion at 45°C and pH 4.5.
• Note: We can also provide conversions to FIP or CDU units upon request, but GDU/g is the primary trade standard in the US market.
Reference Chromatogram of Lactoferrin Reference Substance
While HPLC is not standard for enzyme activity, we provide an SDS-PAGE (Electrophoresis) image in our technical dossier. This visualizes the molecular weight bands, confirming the presence of the active protease fraction (~23-28 kDa) and the purity of the protein isolate.
Stability and Safety
Stability Studies
• Temperature Sensitivity: Bromelain is heat-sensitive. Activity drops significantly if exposed to temperatures >50°C for prolonged periods.
• Storage: Must be stored in a refrigerated environment (2-8°C) or a very cool, dry warehouse (< 20°C) to maintain GDU levels over 24 months.
• Handling: Enzyme dust can cause allergic sensitization. Always wear a mask.
Safety & Handling (MSDS Summary)
• Classification: Respiratory Sensitizer.
• Safety: GRAS (Generally Recognized as Safe) for food use.
• Handling: Avoid inhalation of dust. Wash skin immediately if contact occurs (can cause itching/redness due to protein digestion).
Customer Comments
Product Developer, Sports Nutrition Brand (USA)
2026.04.07
★★★★☆
Quality Manager, Food Processing Co. (Brazil)
2026.04.07
★★★★☆
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FAQ
What is the difference between GDU and MCU?
They are both units of enzyme activity. Roughly, 1 GDU/g ≈ 1.5 MCU/mg. The most common market standard is 2400 GDU/g (High Potency) or 1200 GDU/g (Standard).
Is Bromelain Vegan?
Yes, it is 100% plant-derived from Pineapple stems. It is a perfect vegan alternative to animal-derived enzymes like Trypsin or Pepsin.
Does it work at stomach pH?
Bromelain is unique because it remains active in both the acidic environment of the stomach (pH 3-4) and the alkaline environment of the small intestine, ensuring broad-spectrum protein digestion.
References
1.Brien, S., et al. (2004). Bromelain as a treatment for osteoarthritis: a review of clinical studies. Evidence-Based Complementary and Alternative Medicine.
2.Pavan, R., et al. (2012). Properties and therapeutic application of bromelain: a review. Biotechnology Research International.
3.Maurer, H. R. (2001). Bromelain: biochemistry, pharmacology and medical use. Cellular and Molecular Life Sciences.
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