L-Methylfolate
L-Methylfolate Manufacturer | Bioactive 5-MTHF (CAS: 134-35-0) Headline: L-Methylfolate (5-MTHF) Powder Manufacturer | The Biologically Active Folate (CAS: 134-35-0) Sub-info: Assay ≥ 98.0% | Direct Bioavailability | No Enzymatic Conversion Needed | USP/EP Grade. USP: • Direct Absorption: Bypasses the MTHFR enzyme bottleneck for immediate cellular uptake. • Genetic Solution: The essential form for consumers with MTHFR gene mutations (~40% of population). • Pure Bioactivity: High stereochemical purity (L-Isomer) for maximum efficacy.
Introduction
L-Methylfolate (CAS: 134-35-0), also known as 5-MTHF or (6S)-5-methyltetrahydrofolate, is the primary biologically active form of folate used at the cellular level for DNA replication and the cysteine cycle.
Unlike synthetic Folic Acid, which requires a complex 4-step conversion process in the body, L-Methylfolate (CAS 134-35-0) enters the bloodstream directly and crosses the blood-brain barrier. We provide premium grade L-Methylfolate designed for high-end nutraceutical and pharmaceutical applications, ensuring optimal methylation support without the risk of unmetabolized folic acid accumulation.
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Keywords：
l-methylfolate
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Functions
1.MTHFR Mutation Support
Individuals with the MTHFR C677T or A1298C polymorphism cannot efficiently convert standard folic acid. L-Methylfolate (134-35-0) provides the active methyl group directly, bypassing this genetic limitation.
2.Homocysteine Metabolism
It serves as the critical methyl donor to convert homocysteine into methionine. Maintaining healthy homocysteine levels is vital for cardiovascular health and reducing endothelial inflammation.
3.Neurotransmitter Synthesis
L-Methylfolate is the only form of folate that crosses the blood-brain barrier, acting as a cofactor in the synthesis of serotonin, dopamine, and norepinephrine, supporting mood regulation and cognitive function.
Applications
1.Advanced Prenatal Nutrition
• Why: Ensures the fetus receives active folate for neural tube development, regardless of the mother’s metabolic efficiency.
• Format: Tablets, Capsules (often blended with B12).
2.Clinical Mood Management
• Why: Used as a nutritional adjunct in managing treatment-resistant depression due to its role in neurotransmitter production.
3."Methyl-Free" & Clean Label Supplements
• Why: Preferred by premium brands avoiding synthetic "Folic Acid" on labels in favor of "Bioactive Folate".
Flow Chart
Advanced Stereochemical Control
1.Precursor Preparation: High-purity Folic Acid.
2.Catalytic Hydrogenation: Reduction to Tetrahydrofolate under inert atmosphere.
3.Methylation Reaction: Precise addition of the methyl group to form 5-MTHF (CAS 134-35-0).
4.Chiral Purification: Separation process to isolate the active (6S)-L-isomer from the inactive D-isomer.
5.Crystallization: Forming the pure crystalline powder.
6.Vacuum Drying: Low-temperature drying to strictly control moisture and oxidation.
7.Sieving: Passing through 80 Mesh.
8.Quality Testing: HPLC Analysis & Microbiology.
9.Packaging: Double PE bags inside light-resistant Aluminum drums (Nitrogen flushed).
Quality Standard of Lactoferrin
Product: L-Methylfolate (5-MTHF)
CAS: 134-35-0
| Item | Specification | Result |
| Appearance | White to light yellow crystalline powder | Conforms |
| Assay (HPLC) | ≥ 98.0% | 99.1% |
| Water Content | ≤ 12.0% | 8.5% |
| Specific Rotation | +33.0° ~ +39.0° | +35.2° |
| D-Isomer (Inactive) | ≤ 1.0% | 0.5% |
| Lead (Pb) | ≤ 1.0 ppm | Conforms |
| Arsenic (As) | ≤ 1.5 ppm | Conforms |
| Mercury (Hg) | ≤ 0.5 ppm | Conforms |
| Total Plate Count | ≤ 1000 cfu/g | < 100 cfu/g |
Method of Analysis of Lactoferrin
Chiral HPLC (High-Performance Liquid Chromatography)
• Method: We utilize a specialized Chiral Column to separate the enantiomers.
• Purpose: To strictly verify that the product is the bioactive L-Methylfolate (6S form) and ensure the inactive D-form is minimized (<1.0%). Standard HPLC cannot distinguish these isomers.
Reference Chromatogram of Lactoferrin Reference Substance
(Visual Placeholder: HPLC Graph)
The chromatogram displays a single, sharp peak at the retention time characteristic of L-Methylfolate, confirming high purity and the absence of degradation products or unreacted folic acid.
Stability and Safety
Stability Studies
• Sensitivity: L-Methylfolate (CAS 134-35-0) is sensitive to oxidation, light, and heat.
• Storage Data: Under controlled conditions (2-8°C or cool/dry place <20°C in sealed aluminum containers), the product maintains >98% assay for 24 months.
• Recommendation: We strongly recommend storing this raw material in cold storage to maximize shelf life before formulation.
Safety & Handling (MSDS Summary)
• Classification: Non-hazardous substance.
• Handling: Wear mask and gloves. Avoid dust formation.
• Storage: Keep container tightly closed in a dry and well-ventilated place. Recommended storage temperature: 2 - 8 °C.
Customer Comments
Quality Manager, Nutraceutical Brand (USA)
2026.03.24
★★★★☆
Senior Scientist, Pharma Lab (Germany)
2026.03.24
★★★★☆
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FAQ
What is the difference between CAS 134-35-0 and CAS 151533-22-1?
CAS 134-35-0 refers to the L-Methylfolate (active folate moiety/acid), whereas CAS 151533-22-1 specifically refers to the Calcium Salt form (L-Methylfolate Calcium). We supply high-purity material based on your specific formulation requirements.
Why is L-Methylfolate superior to Folic Acid?
L-Methylfolate is the biologically active form. It does not require conversion by the MTHFR enzyme, making it effective for the ~40% of people with genetic mutations who cannot process regular Folic Acid efficiently.
How should I store this product?
Due to its bioactive nature, L-Methylfolate is sensitive to light and heat. We recommend storing the raw powder at 2-8°C in its original sealed aluminum packaging to ensure maximum potency.
References
1.Scaglione, F., & Panzavolta, G. (2014). Folate, folic acid and 5-methyltetrahydrofolate are not the same bioequivalent. Xenobiotica.
2.Greenberg, J. A., et al. (2011). Folic Acid Supplementation and Pregnancy: More Than Just Neural Tube Defect Prevention. Reviews in Obstetrics & Gynecology.
3.Stahl, S. M. (2008). L-Methylfolate: a novel form of folate for depression. CNS Spectrums.
4.Pietrzik, K., et al. (2010). Folic acid and L-5-methyltetrahydrofolate: comparison of clinical pharmacokinetics and pharmacodynamics. Clinical Pharmacokinetics.
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