P5P pyridoxal phosphate
P5P (Pyridoxal 5'-Phosphate) Manufacturer | Active Vitamin B6 API Headline: Premium P5P (Pyridoxal 5'-Phosphate) Manufacturer | Active Vitamin B6 Coenzyme (CAS: 54-47-7) Sub-headline: 99% High Purity Monohydrate | Direct Bioavailability | No Liver Conversion Needed Unique Selling Proposition (USP): • Metabolically Active: The coenzyme form of Vitamin B6, bypassing the liver conversion step required by standard Pyridoxine HCl. • Superior Absorption: Ideal for individuals with compromised liver function or genetic methylation issues (MTHFR). • Pharmaceutical Grade: Low impurities and high stability suitable for neurological drugs and premium nutraceuticals.
Introduction
Pyridoxal 5'-Phosphate (P5P), CAS No. 54-47-7, is the metabolically active coenzyme form of Vitamin B6. Unlike the common synthetic form (Pyridoxine HCl), P5P does not require enzymatic conversion in the liver to become biologically active.
It appears as a slightly yellow to yellow crystalline powder. As a critical cofactor for over 140 enzyme reactions in the body, P5P is the preferred choice for advanced neurological support formulations, amino acid metabolism, and anti-nausea therapies.
Category：
Keywords：
active
pyridoxal
conversion
Functions
1.Neurotransmitter Synthesis
• Mechanism: Acts as a decarboxylase coenzyme.
• Action: Essential for converting amino acids into key neurotransmitters: Tryptophan to Serotonin, Tyrosine to Dopamine, and Glutamate to GABA. This supports mood regulation and cognitive health.
2.Homocysteine Metabolism
• Mechanism: Cofactor for Cystathionine β-Synthase (CBS).
• Action: Converts toxic homocysteine into cystathionine, reducing cardiovascular risk and supporting healthy methylation pathways (often paired with B12 and Folate).
3.Heme Production & PMS Support
• Mechanism: Key role in hemoglobin synthesis and hormonal balance.
• Action: Helps treat sideroblastic anemia and alleviates symptoms of Premenstrual Syndrome (PMS) by modulating aldosterone and neurotransmitters.
Applications
1.Neurological Supplements
• Application: "Brain Food" capsules and mood support blends.
• Why it works: Direct impact on GABA and Serotonin levels makes it effective for stress, anxiety, and focus without the toxicity risk of high-dose Pyridoxine.
2.Amino Acid & Sports Nutrition
• Application: Protein powders and ZMA (Zinc Magnesium Aspartate) formulas.
• Why it works: B6 is rate-limiting for protein metabolism. P5P ensures maximum utilization of dietary protein for muscle repair.
3.Morning Sickness Relief
• Application: Prenatal vitamins and anti-nausea tablets.
• Why it works: Clinically recognized (often as Diclegis® component) for reducing nausea in pregnancy.
Flow Chart
1.Synthesis: Chemical phosphorylation of Pyridoxal (Vitamin B6 aldehyde) using phosphorus oxychloride.
2.Hydrolysis: Careful hydrolysis to form the crude phosphate ester.
3.Purification: Ion-exchange chromatography to remove inorganic phosphates and unreacted starting material.
4.Crystallization: Controlled cooling to form the stable monohydrate crystal.
5.Centrifugation & Washing: Removal of residual solvents.
6.Drying: Low-temperature vacuum drying (Heat sensitive).
7.Sieving: Passing through 60-80 mesh screens.
8.Packaging: 1kg/5kg Aluminum tins or light-resistant drums.
Quality Standard of Lactoferrin
Product: Pyridoxal 5'-Phosphate Monohydrate (USP/EP)
CAS: 54-47-7 (Anhydrous: 41468-25-1)
| Item | Specification | Result |
| Appearance | Slightly yellow to yellow crystalline powder | Conforms |
| Assay (on dry basis) | 98.5% - 101.0% | 99.6% |
| pH (0.5% solution) | 2.5 - 3.5 | 3.1 |
| Loss on Drying | 8.0% - 11.0% (Monohydrate) | 9.8% |
| Water (KF) | ≤ 10.0% | 9.5% |
| Heavy Metals | ≤ 10 ppm | < 5 ppm |
| Chloride (Cl) | ≤ 0.05% | 0.02% |
| Microbial Limit | TAMC < 1000 CFU/g | Conforms |
Method of Analysis of Lactoferrin
Test Method: UV Spectrophotometry / HPLC
• Assay: Measured by UV absorption at 388 nm in phosphate buffer (pH 7.0).
• Identity: IR spectrum must match the reference standard.
• Purity: HPLC is used to detect related substances like free Pyridoxal or Pyridoxine.
Reference Chromatogram of Lactoferrin Reference Substance
(Description)
The HPLC profile displays a clean main peak for P5P. The retention time is distinct from Pyridoxine HCl. Impurities (free pyridoxal) should be <0.5%.
Stability and Safety
Stability Studies
• Light Sensitivity: Extremely sensitive. UV light causes rapid photodegradation (turning the powder brown/orange).
• Thermal Stability: Sensitive to heat.
• Shelf Life: 24 Months when stored in strictly light-proof, cool containers (< 25°C).
Safety & Handling (MSDS Summary)
• Classification: Non-hazardous active ingredient.
• Handling: Avoid inhalation. Protect from light during processing.
• Storage: Protect from light. Store in a cool, dry place.
Customer Comments
Product Developer, EliteSports Nutrition (UK)
2026.03.05
★★★★☆
Quality Manager, NeuroBio (USA)
2026.03.05
★★★★☆
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FAQ
Why use P5P instead of regular Vitamin B6?
Regular B6 (Pyridoxine) must be converted by the liver into P5P to be used. Many people have compromised liver function or genetic variations (like MTHFR) that hinder this conversion. P5P skips this step, offering direct bioactivity.
Is it water soluble?
Yes, P5P is soluble in water (approx 5mg/mL), making it suitable for liquid drops and beverages, though it must be protected from light in the final packaging.
Does it cause neuropathy like high-dose B6?
While toxicity is possible with any vitamin, P5P is generally considered safer because it doesn't need to be processed by the liver and doesn't build up in the blood in the same way unconverted Pyridoxine can.
References
1.Spinneker, A., et al. (2007). "Vitamin B6 status, deficiency and its consequences--an overview." Nutrition & Metabolism.
2.Clayton, P.T. (2006). "B6-responsive disorders: a model of vitamin dependency." Journal of Inherited Metabolic Disease.
3.United States Pharmacopeia (USP) Monograph: Pyridoxal 5'-Phosphate.
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