4-Hydroxyisoleucine
4-Hydroxyisoleucine Powder | Natural Insulin Sensitizer Headline: High-Purity 4-Hydroxyisoleucine Manufacturer | 98% from Fenugreek (CAS: 55399-93-4) Sub-info: Source: Trigonella foenum-graecum (Fenugreek Seed) | Assay: 98% | Non-Protein Amino Acid USP: Glucose Metabolism Support • Glycogen Resynthesis • Non-GMO Plant Extraction
Introduction
4-Hydroxyisoleucine (4-HIL) is a naturally occurring non-protein amino acid found in fenugreek seeds (Trigonella foenum-graecum). The product is obtained from botanical raw materials through extraction and purification processes, without synthetic modification.
Scientific studies indicate that 4-Hydroxyisoleucine supports glucose metabolism by promoting insulin secretion in a glucose-dependent manner. This characteristic allows physiological regulation of postprandial blood glucose while minimizing the risk of hypoglycemia.
Category：
Keywords：
4-hydroxyisoleucine
fenugreek
trigonella
Functions
1.Glucose Regulation (Insulin Sensitizing)
4-HIL has been shown to act on pancreatic beta cells, enhancing insulin secretion when blood glucose levels are elevated. This glucose-dependent response contributes to improved glycemic control without excessive insulin release.
2.Sports Nutrition (Glycogen Recovery)
By improving insulin sensitivity, 4-HIL supports the uptake of glucose and amino acids into skeletal muscle cells. This mechanism is beneficial for post-exercise glycogen resynthesis and recovery, making it suitable for sports nutrition formulations..
3.Lipid Metabolism
Research suggests that 4-HIL may help reduce plasma triglycerides and free fatty acids, contributing to a healthier lipid profile and supporting metabolic balance.
Applications
Diabetes Management Supplements:
• Blood Sugar Formulas: A key ingredient in "Glucose Support" capsules, often combined with Berberine and Chromium.
Sports Nutrition:
• Post-Workout Recovery: Added to carbohydrate-protein blends (mass gainers) to maximize nutrient uptake into muscles (anabolic effect).
• Creatine Transport: Acts as an insulin mimic to improve Creatine absorption without excessive sugar intake.
Weight Management:
• Helps regulate appetite and carbohydrate metabolism.
Flow Chart
Fenugreek Seed Selection → Crushing → Solvent Extraction (Ethanol/Water) → Filtration → Concentration → Purification (Ion Exchange Chromatography) → Crystallization → Vacuum Drying → Milling → Quality Control (HPLC) → Packaging
Note: Our proprietary purification removes the characteristic strong odor of Fenugreek (Sotolon), resulting in a neutral-smelling powder suitable for flavored powders.
Quality Standard of Lactoferrin
| Item | Specification | Result |
| Appearance | White or off-white crystalline powder | Conforms |
| Assay (HPLC) | ≥ 98.0% | 98.5% |
| Loss on Drying | ≤ 1.0% | 0.4% |
| Heavy Metals | ≤ 10 ppm | Conforms |
| Lead (Pb) | ≤ 2.0 ppm | Conforms |
Method of Analysis of Lactoferrin
We use HPLC with ELSD (Evaporative Light Scattering Detector) or UV derivatization to quantify 4-Hydroxyisoleucine, as it lacks a strong UV chromophore.
• Isomer Specificity: Our method ensures the presence of the bioactive (2S,3R,4S) isomer found naturally in Fenugreek.
Reference Chromatogram of Lactoferrin Reference Substance
(Imagine a clean HPLC peak) The chromatogram displays a single, sharp peak at the specific retention time. Impurities such as Trigonelline or other amino acids are effectively removed to <2%.
Stability and Safety
Stability Studies
• Shelf Life: 24 months.
• Hygroscopicity: Moderately hygroscopic.
• Storage: Store in a cool, dry place. Keep container tightly closed. Stability data shows <2% degradation after 24 months at 25°C/60% RH.
Safety & Handling (MSDS Summary)
• Classification: Non-hazardous botanical isolate.
• Safety: No observed toxicity at functional doses (200-500mg/day).
• Handling: Wear standard PPE.
Customer Comments
Product Manager, US Supplement Brand
2026.02.13
★★★★☆
Formulator, UK Sports Nutrition Co.
2026.02.13
★★★★☆
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FAQ
What is the source of your 4-Hydroxyisoleucine?
It is extracted from Fenugreek seeds (Trigonella foenum-graecum). We do not use synthetic sources. This ensures the correct stereochemistry for biological activity.
Does it smell like Fenugreek?
Raw Fenugreek powder has a strong "maple syrup" or "curry" odor due to Sotolon. Our 98% Purity 4-Hydroxyisoleucine is highly purified and has very little to no odor, making it perfect for flavor-sensitive applications.
What is the recommended dosage?
For blood sugar support or sports recovery, clinical studies often use doses ranging from 100mg to 500mg per day, typically taken before meals or post-workout.
References
1.Broca, C., et al. "4-Hydroxyisoleucine: experimental evidence of its insulinotropic and antidiabetic properties." American Journal of Physiology-Endocrinology and Metabolism (1999).
2.Sauvaire, Y., et al. "4-Hydroxyisoleucine: a novel amino acid potentiator of insulin secretion." Diabetes (1998).
3.Ruby, B. C., et al. "The addition of fenugreek extract (4-hydroxyisoleucine) to glucose feeding increases muscle glycogen resynthesis after exercise." Amino Acids (2005).
4.Jetté, L., et al. "4-Hydroxyisoleucine: a plant-derived treatment for metabolic syndrome." Current Opinion in Investigational Drugs (2009).
5.Avalos-Soriano, A., et al. "4-Hydroxyisoleucine from Fenugreek (Trigonella foenum-graecum): Effects on Insulin Resistance and Gastrointestinal Function." Molecules.
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